Tm Calculator icon

This tool calculates the Tm of primers and estimates an appropriate annealing temperature when using different DNA polymerases. How to use this calculator

Quickly find the right annealing temperature for Platinum SuperFi DNA polymerase (also works for SuperScript IV One-Step RT-PCR Kit), Phusion and Phire DNA polymerases.

Important note: If the PCR primer contains desired mismatches, e.g., for creating a mutation or a restriction site, make sure to calculate the Tm only for the correctly matched sequence

The T m calculator is not required for Platinum II Taq DNA Polymerase, Platinum SuperFi II DNA Polymerase, and Platinum Direct PCR Universal Master Mix due to their buffers specially formulated for universal annealing at 60°C for primers.

Our standard Taq-based calculator is being upgraded and will be back online soon.

1. Select your DNA polymerase